Salmon Sperm Extract /Polydeoxyribonucleotide (PDRN)

Core Component——DNA

Production Mechanism

• Hepatogenesis: Nucleic acids are produced from small simple compounds, starting with the synthesis of nucleobases (purines, pyrimidines), etc..

• Supplementation from exogenous sources: Synthesize nucleic acids by digestion and absorption of foods containing nucleic acids.

Importance to the Human Body

• The human body consists of 60 trillion cells, and nucleic acid is the basic component of cells.

• The cellular metabolic cycle of the body is 120 days, and sufficient nucleic acid DNA can accelerate cellular metabolism, improve skin condition, and restore cellular vitality.

• Endogenous and exogenous sources of nucleic acids dominate the normal division, proliferation, and metabolism of human cells.

Why choose Salmon Sperm Extract?

DNA Damage

• DNA damage caused by radiation, pollution, and chemical toxins in the environment

• Decreased ability to synthesize nucleic acids due to the aging of the human body

Exogenous supplementation

The human body is within the range of 2-2.5 g per day, and the recommended daily intake of nucleic acids for normal adults is about 0.6-1.2 g, with a maximum of 2 g.

As shown in the table below, the content of nucleic acids in food is low, and overtaking can also cause a risk of cholesterol overload.

Application History

As early as 1999, there was already a DNA nucleic acid supplement focusing on anti-aging in Japan, using salmon sperm extract as an ingredient.

PDRN: Dual-Spin A2A™ Technology Gives products powerful efficacy

The double helix structure has better receptor specificity; namely, it activates the adenosine A2A2 receptor. 0.1% of this product activates A2AR+23.89%.

In vitro experiments show that it can significantly enhance the antioxidant and self-repair capabilities of skin tissue.

Antioxidant enzyme SOD↑ +98.67% Catalase CAT↑ +200.35%

Human clinical trial: Dosage: 300 mg/day, once daily;

60 participants aged 30–50 years (two groups) Forehead wrinkle area decreased by -20.64%↓; dermal collagen density increased by +13.27%↑;

Skin radiance increased by +23.72%; Stratum corneum moisture content increased by +24.38%↑

Absorption of Nucleic Acids in the Body

Mechanism of action of dual-spin A2A™  PD RN

Adenosine A2A receptors belong to the G protein-coupled receptor family and are highly expressed on immune cells. They participate in inflammatory responses, immune regulation, and inflammation suppression, and cell renewal and metabolism by regulating immune cells.

The mechanism by which salmon DNA participates in the adenosine A2A receptor pathway ultimately leads to epidermal renewal and repair.

Mechanism verification:  PDRN-C activates the adenosine A2AR receptor

• Experimental Model: Fibroblast proliferation model

• Experimental Method: Except for the control group, the  corresponding test samples were added to the cell culture medium and incubated for 24 h.

• Experimental Concentration: 0.05%, 0.1%

• Detection Instrument: Inverted fluorescence microscope

• Detection Index: A2AR content

• Detection Method: Image-Pro Plus 6.0 image analysis software

Activation of A2A receptors can reduce inflammatory infiltration, promote endothelial cell proliferation and migration, reduce VEGF production, and stimulate fibroblast differentiation and maturation, thereby accelerating the repair process.

Experimental conclusions: 0.05% and 0.1% of the di-swirl A2A™ PDRN can activate adenosine A2AR receptors, with activation rates of 16.51% and 23.89%, respectively.

Note: BC is the blank control group; blue marks the cell nuclei, yellow marks A2AR, and the brighter the yellow fluorescence, the higher the A2AR content.

Efficacy Mechanism of DNA-Na

Protection against UVB damage

Increased keratinocyte survival rate and reduced apoptosis rate; decreased ROS content.

Delaying endogenous aging

Promoting type I collagen, ATP significantly enhances SOD and CAT activity.

Improve inflammatory response

Significantly inhibits IL-6 and enhances fibroblast viability.

Visualization enhances youthfulness. energy

Completely eliminate crow's feet and forehead wrinkles; improve skin radiance and elasticity.

Salmon Sperm Extract Protects Against UVB Damage—IN VITRO

0.25% salmon sperm extract increased the survival of UVB- injured keratinocytes by 63%; reduced the rate of apoptosis by 70%; and reduced the nuclear damage of keratinocytes by 75%!

Salmon Sperm Extract Protects Against UVB Damage - IN VITRO

In Picture B, the proportion of red fluorescence in stained cells is high with unclear cell contour and many apoptotic cells.

In picture B, the cell nuclei have a deeper color, many fragments, and more damage.

Salmon Sperm Extract Protects Against UVB Damage - Reduction of  ROS Content

Note: ROS is marked in green. If the mark is brighter, the content is higher, and the oxidative damage to the cell is more severe.

Improve inflammatory response—Significantly inhibits IL-6

Dual-Swirl A2A™ PD RN can enhance fibroblast activity, inhibit IL-6 secretion, and have anti-inflammatory effects .

Promote fibroblast proliferation

Promote type I collagen

• Objective: anti-aging

• Cell: human primary fibroblasts (P8)

• Instrument: inverted fluorescence  microscope

• Test Sample:  Double  helix A2A ™   PD RN

• Test groups:  Blank,  Experimental

• Test indicator: COL I fluorescence intensity

Note: Blue labeling represents cell nuclei, while purple labeling represents type I collagen (COLI).

Promote ATP production.

• Objective:Anti-aging efficacy of test samples

• Cell: human primary fibroblasts (P8)

• Instrument: inverted fluorescence  microscope

• Test Sample:   Double  helix A2A ™   PD RN

• Test groups:  Blank,  Experimental

• Test indicator: ATP fluorescence intensity

Note: Blue labeling represents cell nuclei, while red labeling represents ATP.

Reducing exogenous oxidative stress damage Zebra fish experiment

• Objective:Anti-aging efficacy of test samples

• Cell ：acrylamide-induced zebrafish embryos

• Instrument：Stereo microscope

• Test Sample:  Double helix A2A ™  PDRN

• Experimental groups: Blank group, model group, sample group, control group

• Detection indicators: Hatching rate, yolk sac area

OriginBio

A reliable one stop nutrition supplement contract manufacturing partner